Abstract:Background:This study investigated the antibiofilm potential of copper oxide nanoparticles (CuO NPs) synthesized using Muntingia calabura leaf extract against Pseudomonas aeruginosa. Methods:Environmental samples were collected from water pipelines in Floridablanca, Pampanga. P. aeruginosa was isolated and confirmed using VITEK. CuO NPs were green-synthesized using M. calabura leaves as a reducing agent. Nanoparticles were characterized by Field Emission Scanning Electron Microscopy (FE-SEM) for size and morphology. Minimum Inhibitory Concentration (MIC) and antibiofilm activities were assessed via the microbroth dilution method, with ciprofloxacin as the control. NPs were tested at 25%, 50%, 75%, and 100% concentrations, and biofilm inhibition was quantified using UV-Vis spectrophotometry at OD600. Results:M. calabura-CuO NPs showed concentration-dependent inhibition of P. aeruginosa. Significant biofilm reduction was observed at 25% and 100% concentrations, with the MIC determined at 25 µg/mL. In some cases, the NPs matched the efficacy of ciprofloxacin. Conclusion:M. calabura-CuO NPs effectively inhibited P. aeruginosa biofilms, particularly at 25% and 100% concentrations. The MIC of 25 µg/mL suggests promising antimicrobial potential. These green-synthesized nanoparticles could serve as an alternative or adjunct to conventional antibiotics like ciprofloxacin. Status of Study The study is finished and has passed ethical considerations
Keywords: Antimicrobial Resistance (AMR), Keywords: Copper oxide nanoparticles, Pseudomonas aeruginosa, biofilm inhibition, minimum inhibitory concentration, Muntingia calabura
