Abstract ID: 171
Authors:
Abdulmumin Yunusa
Iman Usman Haruna
Zainab Rabiu
Umma Lawan
Sadiya Warshu Haruna
Murtala Muhammad
Anas Abba
Adamu Alhassan Jibril
Mujahid Nura Sani
Rabi’atu Kabir Mustapha
Affiliations:
Department of Biochemistry, Faculty of Basic Medical Sciences, Northwest University, Kano, Nigeria; Department of Obstetrics and Gynaecology, College of Medicine, Northwest University, Kano, Nigeria; Department of Community Medicine, College of Health Sciences, Northwest University, Kano, Nigeria; Department of Biochemistry, Aliko Dangote University of Science and Technology, Wudil, Kano State, Nigeria; Department of Microbiology, Aliko Dangote University of Science and Technology, Wudil, Kano State, Nigeria; Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Bayero University, Kano, Nigeria; Department of Biochemistry, Bayero University, Kano, Nigeria; Department of Chemistry, Faculty of Science, Northwest University, Kano, Nigeria.
Abstract:Background Non-communicable diseases (NCDs) such as cardiovascular, hepatic, renal disorders, diabetes, and cancer are a growing public health challenge in Nigeria. Despite the widespread use of medicinal plants in traditional Nigerian medicine, limited scientific validation exists to support their clinical efficacy. Natural product-based therapeutics offer potential advantages in reducing toxicity and enhancing drug safety. Aim: The study aims to identify, isolate, and evaluate the bioactivity and mechanisms of action of novel compounds from selected Nigerian medicinal plants for the management of NCDs. The Specific objectives are: Isolation and characterization of lead compounds using LC-MS and NMR techniques (¹³C, ¹H, HSQC, COSY, NOESY, DEPT). In vivo antioxidant evaluation of lead compounds in albino rats. In vivo efficacy assessment of the compounds against NCD models in Wistar rats. In-silico evaluation of mechanisms via molecular docking and ADMET profiling. In vitro plaque reduction, neutralization, and cytotoxicity assays. Toxicological profiling of the identified lead compounds. Method sselected plant extracts will undergo bioassay-guided fractionation to isolate active compounds. Structural elucidation will be carried out using FTIR, LC-MS, and NMR. The efficacy of these compounds will be tested in rat models of NCDs, with analyses of key biochemical markers. Mechanistic studies include molecular docking and dynamic simulations. Toxicity and ADME profiles will be assessed using in silico tools and in vitro assays. Ethical approval has been obtained from the institutional animal ethics committee and the Kano State Ministry of Health. Data will be analyzed using SPSS, with significance set at p < 0.05. Expected Outcomes Isolation of novel, non-toxic bioactive compounds effective against NCDs. Insight into their mechanisms of action. Development of potential candidates for further preclinical development. Status of the study Ongoing.
Keywords: Non-Communicable Diseases (NCDs), Non-communicable diseases, Medicinal plants, in-vivo, in-vitro, bioinformatics
